We are an enthusiastic and collaborative group of scientists. For us, caves are perfect natural laboratories and we study cave animals from sponges to vertebrates.

We integrate experimental procedures across biological disciplines, from molecular to organismal, and apply diverse methodological approaches both in the lab and in the field to answer important questions in evolutionary biology.

Helena Bilandzija

Helena Bilandzija

PI

My research focuses on identifying molecular mechanisms underlying adaptive evolution in cave-dwelling animals.

I have started my lab at Rudjer Boskovic Institute in Zagreb, Croatia in 2019, where I plan to use comparative evo-devo approaches to study the process of colonization and adaptation to cave environments.

Researchgate profile

Google scholar profile

Marina Horvatiček

Marina Horvatiček

Postdoc

Biologist with a wide spectrum of interests, from investigating epigenetics and diabetes metabolism during human pregnancy to investigating the evolution and epigenetics of cave fishes. As the latest member of the Evo Dark group in a postdoc position, I'm involved in experiments on the teleost fish Astyanax mexicanus, where I contribute to the research of molecular mechanisms undergoing phenotypic plasticity.

Researchgate profile

Jana Bedek

Jana Bedek

Postdoc

My current focus in the lab is understanding the adaptations of various invertebrates to a subterranean lifestyle.

I am also interested in the taxonomy of cave-dwelling Isopoda and have completed a PhD on the taxonomy and molecular phylogeny of the southern European genus Alpioniscus.

I am interested in the conservation of cave fauna in the Dinaric Karst as well.

Researchgate profile

Lada Jovović

Lada Jovović

PhD student

I am a molecular biologist who has always been passionate about evolution as an approach to explaining biodiversity from molecules to entire ecosystems.

For my PhD, I am studying the phenomenon of pigment loss as a common adaptation in cave dwellers. A lot of research has been done on melanin, but I am particularly interested in ommochromes - generally less studied biological pigments first described in the eyes of Drosophila.

I would like to determine the precise molecular mechanisms that lead to albinism as a result of ommochrome deficiency.

In addition, I would like to determine if the loss of ommochromes, similar to the loss of melanin, may be an evolutionarily favored adaptation in cave dwellers because it can enable for a shift of resources (substrates and/or enzyme machinery) to biological processes that are more useful in the stressful and harsh cave environment.

Researchgate profile

Magdalena Grgić

Magdalena Grgić

PhD student

I am investigating the role and possible alterations of the phenoloxidase system (PO) in the process of pigment loss.

Since PO is an important component of invertebrate immunity, changes in its activity could also contribute to the evolution of some other physiological and behavioural adaptations to cave life.

To understand these processes, I am working on the genus Congeria (Bivalvia, Dreissenidae) - the world's only stygobiont bivalve.

I also participate in the Identification of stygobiotic fauna of Plitvice Lakes National park project.

Researchgate profile

Lucija Kauf

Lucija Kauf

PhD student

During my studies, I developed an interest in cave animals and evolution, which eventually led me to join this group.

I am working on identifying the molecular basis of pigmentation in planarians and the mechanisms of its loss in cave-dwelling species. Depending on the species, this group of animals can have melanin, ommochrome, or porphyrin pigments, and pigmented species often have more than one pigment type. On the other hand, several different species have colonized caves and developed albinism. I will identify the pigment type in surface species and use comparative transcriptomic, genomic, and biochemical approaches to find the cause of the cessation of pigment synthesis in their albino cave-dwelling relatives.

Marko Lukić

Marko Lukić

Expert associate

From my first caving trip, I was fascinated by the strange ecosystems and animals that live in caves.

I became interested in the taxonomy of Collembola and their morphological and behavioral adaptations to the cave environment.

My PhD thesis focused on the systematics and biogeography of the European Collembola genus Verhoeffiella, discovering the radiation of this genus in Dinarid caves.

My role in the lab is to take care of the invertebrate and fish facility, cultivate and breed numerous species under laboratory conditions, and design the innovative experimental setups for the lab projects.

I enjoy observing and filming cave animals and also work on popularizing and preserving cave fauna within the Dinarides.

Researchgate profile

Lucia Ilijić

Lucia Ilijić

Associate

In this lab i completed my masters' thesis, which focused on investigating phenotypic variability of retinal morphology in surface and subterranean populations of the freshwater fish Telestes karsticus.

My current role is taking care of the group’s invertebrate and fish facility.

Manuela Žugaj

Manuela Žugaj

Graduate student

I am currently doing masters degree in experimental biology and I joined this team because of my interest in molecular genetics. I was working on phenotipic variability of cave and surface populations of freshwater crustacean Asellus aquaticus. For my masters thesis I am working on epigenetics on the same species.

Eva Marković

Eva Marković

Graduate student

As a molecular biology master's student, I am currently working on my thesis project in the Bilandžija group. I have always been fascinated by evolutionary biology and the mechanisms of adaptation, particularly in extreme environments.

I am deeply interested in integrating environmental factors as drivers of evolution with the molecular mechanisms that underlie these processes. This is the reason I joined this lab, where I'm able to explore the phenomenon of pigment loss in the isopods from the Proasellus genus and help decipher the evolutionary cause of the phenomenon.

Martina Kuzman

Martina Kuzman

Graduate student

Throughout my studies molecular genetics was a field that always peaked my interest which led me to join this group. 

I'm an experimental biology master's student and for my master's thesis I'm working on Scarlet gene knock-down with RNA interference in Isopods; Proasellus coxalis and Asellus aquaticus

Lucija Tomić

Lucija Tomić

Graduate student

I am a graduate student in experimental biology and currently doing my master's within this project. We are doing research in metabolic rates through oxygen consumption between surface and subterranean asellid crustaceans.

As I enhanced my practical skills in lab, I ended up being more interested in cave-dwelling organisms and its' adaptations to caves.

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